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Plasmids used in this work
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Plasmids used in this work

Journal: Microbial Cell Factories

Article Title: Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering

doi: 10.1186/s12934-019-1127-8

Figure Lengend Snippet: Plasmids used in this work

Article Snippet: Five CYP-coding genes ( BPLO from Betula platyphylla , CrAO from Catharanthus roseus , MtAO12 from Medicago truncatula , VvAO15 and VvAO17 from Vitis vinifera ), five CPR-coding genes ( ATR2 from Arabidopsis thaliana , CrCPR from C atharanthus roseus , GuCPR from Glycyrrhiza uralensis , LjCPR from Lotus japonicas and MTR from Medicago truncatula ), four genes coding enzymes related to NADH or NADPH generation ( EMC from Mucor circinelloides , EMT from Mortierella alpina , Gapc from Kluyveromyces lactis and Rtme from Rhodotorula toruloides NP11 ) and sfGFP were codon optimized and synthesized by GenScript (Nanjing, China) and listed in Additional file : Table S3.

Techniques: Clone Assay